Single molecular DNA/RNA sequencing with microscopy
نویسندگان
چکیده
منابع مشابه
Single Molecule Fluorescence Microscopy and its Applications to Single Molecule Sequencing by Cyclic Synthesis
1.0. Introduction 2.0. Background 2.1. Single Molecule Detection 2.2. Total Internal Reflection 2.3. FRET Theory 3.0. DNA Sequencing by Cyclic synthesis 3.1. Motivation 3.2. Surface Treatment 3.3. Polymerase Kinetics 3.4. Sequencing Strategies 3.4.1. Cyclic Synthesis using FRET 3.4.2. Real Time Imaging 3.4.3. Non FRET Imaging 3.4.4. Cleavable Linkers 3.4.5. Cleavable Terminators 3.4.6. Multi-Co...
متن کاملScanning tunneling microscopy experiments on single molecular landers.
Molecular landers are molecules comprising of a central rigid molecular wire maintained above a metallic surface by organic spacers, which allows specific ultrahigh vacuum-scanning tunneling microscopy experiments to be performed at the single-molecule level. The understanding of the molecule-surface interactions, intramolecular mechanics, and the possibility to perform extremely precise tip-in...
متن کاملSIGNATURE: a single-particle selection system for molecular electron microscopy.
SIGNATURE is a particle selection system for molecular electron microscopy. It applies a hierarchical screening procedure to identify molecular particles in EM micrographs. The user interface of the program provides versatile functions to facilitate image data visualization, particle annotation and particle quality inspection. The system design emphasizes both functionality and usability. This ...
متن کاملVisualizing single molecular complexes in vivo using advanced fluorescence microscopy.
Full insight into the mechanisms of living cells can be achieved only by investigating the key processes that elicit and direct events at a cellular level. To date the shear complexity of biological systems has caused precise single-molecule experimentation to be far too demanding, instead focusing on studies of single systems using relatively crude bulk ensemble-average measurements. However, ...
متن کاملA Single Machine Sequencing Problem with Idle Insert: Simulated Annealing and Branch-and-Bound Methods
In this paper, a single machine sequencing problem is considered in order to find the sequence of jobs minimizing the sum of the maximum earliness and tardiness with idle times (n/1/I/ETmax). Due to the time complexity function, this sequencing problem belongs to a class of NP-hard ones. Thus, a special design of a simulated annealing (SA) method is applied to solve such a hard problem. To co...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Asian Journal of Pharmaceutical Sciences
سال: 2016
ISSN: 1818-0876
DOI: 10.1016/j.ajps.2015.11.113